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KMID : 1094720070120020147
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 2 p.147 ~ p.151
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The effects of nonionic surfactants on the pretreatment and enzymatic hydrolysis of recycled newspaper
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Kim Hyun-Joo
Kim Sung-Bae
Kim Chang-Joon
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Abstract
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The effects of surfactants on the pretreatment and enzymatic hydrolysis stages of recycled newspaper processing were examined. Newspaper substrate was pretreated with surfactants at 40°C and 400 rpm for 1 h, and the enzymatic digestibilities of the pretreated substrate were compared. NP-20 was 10?20% more effective as a surfactant than Tween-20 and Tween-80. To investigate the effects of the surfactants on the subsequent enzymatic hydrolysis stage, the newspaper was pretreated with NP-20 and then hydrolyzed in the presence of TW-20 or TW-80. TW-80 showed an approximate 7% higher digestibility than TW-20. The surfactant effect on the hydrolysis of the untreated newspaper was significant, whereas the surfactant effect on the hydrolysis of the surfactant-pretreated newspaper was marginal. When the digestibilities of the pure cellulose substrates (α-cellulose and filter paper) were examined, markedly different surfactant effects were observed. In contrast to the newspaper substrate, the surfactant-pretreated pure cellulose substrates had a significant effect on digestibility when they were hydrolyzed in the presence of a surfactant, indicating that the surfactant effect on digestibility is highly dependent on substrate type.
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KEYWORD
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newspaper, pretreatment, enzymatic hydrolysis, surfactant, digestibility, α-cellulose
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